Burnt is one of the common wound in the world and using modern methods such as use of cell therapy and nanoparticles can be considered as a non-aggressive and effective strategy in the treatment of these wounds. Cell therapy involves the replacement of stem cells or tissues made from stem cells for various diseases and injuries. Also, Nanoparticles with their unique features have different biological and biomedical usages in this regard. The aim of this study is investigating the effects of using graphene quantum dots (GQDs) associated embryonic fibroblast cells on treating third degree burnt in Wistar rats. In this experiment, stem cells were obtained by isolating embryonic fibroblast cells from 13-day fetal NMRI mice. MTT test was performed to determine the dose of nanoparticle and Dil color, which resulted in a suitable dose of 100µg/ml nanoparticle and a suitable concentration for 1µg/ml Dil color. For this study, 40 Wistar rats were burned by heating a metal rod of 1cm in diameter and then randomly divided into two groups of control (10 rats) and treatment (30 rats). The treatment group was divided into three groups (each group of 10 rats) of daily injection of GQDs nanoparticle with a concentration of 100µg/ml, cell therapy, and cell therapy+ nano. Cells (passage 3) were labeled with Dil color, and 106 cells were injected into each rat subcutaneously. Each rat was kept separately in a cage for up to 40 days with unlimited access to water and food. On days 20 and 40, clotting was performed with chloroform and histological sections were prepared by staining Hematoxylsene-Eosin (H&E) and Trichromosone done for microscopic examination. Macroscopic and microscopic observations showed that in the treatment groups, the recovery in treated groups was higher than the control group. Also, cell therapy and GQDs injection with 100µg/ml concentration and simultaneous injection of cell therapy + nano accelerated the wound healing process and the cell therapy + nano was significantly more effective than nanoparticles and cell injection alone after 20 and 40 days. Histological studies indicated a significant increase in angiogenesis, number of cells, collagen synthesis, thickness of skin layers, and ultimately acceleration wound healing in treatment samples compared to controls. Based on these results, it seems that the same treatment methods used in this study, significantly, accelerate the repair of skin lesions in animal model.