In this research, dipyridamole is preconcentrated and determined with magnetic molecularly imprinted polymer in pharmaceutical formulations and biological fluids such as tablet, serum and plasma .
In this purpose, Fe3O4 nanoparticles were synthesized as magnetic particeles, then it was modified with amine groups using APTES. Finally by using EGDMA as cross-linker and in the precence of dipyridamole as template, polymerization was carried out. To remove the template molecules from MMIP, as-synthesized MMIPs were washed with acetic acid/ethanol (7:3, V/V). The final product was used as a selective sorbent for preconcentration and determination of dipyridamole with dispersive-micro-solid phase extaction.
Response surface methodology (RSM) was applied to survey and optimize the operating factors affecting the determination of dipyridamole to increase the efficiency of MMIP extraction. The factors included in our design were sorbent dosage (w), the volume of eluent (v) and time of desorption(t) .
HPLC is one of the sensitive and selective methodologies for analysis of drug that was used in this work.The highest adsorption capacity for dipyridamole is 2.82 mgg-1. linear range obtained for dipyridamole is 0.008-200 µg L-1, detection limit and quantification limit are 0.003 µg L-1 and 0.009 µg L-1, respectively. the relative standard deviation for method was obtained with interday in concentration 5, 50 and 150 respectively 3.26%, 2.09%, 3.41% and for intraday in concentration 5, 50 and 150 respectively 1.42%, 3.84%, 3.16%.

 In this work, an efficient, sensitive and selectivity analytical method solid-phase
microextraction (SPME) fiber on the basis of monolithic moleculary imprinted
polymer-GO/IL coupled to reverse phase high performance liquid chromatography
(RP-HPLC) – UV detection analysis was developed for extraction and determination of
sulfamethoxazole and sulfamethazine in food samples such as chiken muscle, egg and
milk. In this study, Graphene oxide was synthesized according to hummer method. In
next step Go was coated with Ionic Liquid by reflux method in the presence of DMF
solvent. Followed Amount determined of template molecule (Sulfamethazine) was
dissolved in aporogen solvent (acetonitrile) and than functional monomer (MAA),
cross-linking monomer (EGME) and intionar (AIBN) were added respectively. Than
Go-IL added in above solution. After the 1mL of the mixture was transferred into small
glass tube and deoxygenized under nitrogen gas. Followed, the glass tube is filled with
the polymerization mixture and The filled capillaries are introduced in an oven and
polymerization takes place typically at temperature 60 ◦C for 24 h of time. To obtain
optimum extraction performance, several extraction parameters including extraction
and desorption solvent, stirring rate, extraction and desorption time were investigated
and discussed. Under the optimized extraction conditions, the limits of detection (S/N =
3) of the proposed method were 0.08 and 0.06 µg L−1
, for sulfamethoxazole and
sulfamethazine, respectively. linear relationship was achieved in the range of 0.1-40 µg
L
−1
for analytes.
.

Ciprofloxacin belongs to fluoroquinolones antibiotics which are used to treat both, gram-negative and gram-positive bacetria infections. Mostly, it is used to treat intra abdominal, respiratory tract and urinary tract infections. It functions by inhibiting DNA gyrase enzymes necessary to separate bacterial DNA, thereby inhibiting cell division. This drug may cause some side effects such as, arthropathy in children or hysteria in some patients.
In this study, Fe3O4 nanoparticles were synthesized as magnetic particeles, then it was coated with Si. Surface of silica-coated nanoparticles were modified with amine groups. Finally by using EGDMA as cross-linker and in the precence of ciprofloxacin as template, polymerization was carried out. To separate the template molecules from MMIP, prepared MMIPs were washed with methanol/acetic acid (9:1, V/V). The final product was used as a selective solid-phase extractor for ciprofloxacin.
One of the properties which has made MMIP to be more efficient, is its magnetic nature, because it can be easily collected from the environment by an external magnetic field. HPLC/UV was used as an efficient and sensitive technique for determination of ciprofloxacin.
Conditions affecting the determination of ciprofloxacin, were optimized to increase the efficiency of MMIP extraction. Synthesized MMIP showed a high tendency to adsorb ciprofloxacin rather than similar molecules. The maximum adsorption capacity was 166.2 mg g−1. Experiments exhibited a linear range of 1-10000 ng mL−1 for Ciprofloxacin with the correlation coefficient (r) of 0.9986. Evaluated LOD and LOQ were 0.28 and 0.92 ng mL-1 respectively.
 

In this work, Chitosan / nano alumina biocomposite supported by silver nanoparticles was prepared by chemical precipitation method. The synthesized composite was characterized by FTIR spectroscopy and scanning electron microscopy analysis. The adsorption of dye on the adsorbent with respect to contact time, pH and adsorbent dosage was investigated to provide more information about the adsorption characteristics of biocomposite. Optimized values of adsorbent nanoparticle dosage and pH for acid red 14 adsorption were found as 0.15 g, and 5, respectively. The optimum removal efficiency of the nanoadsorbent for acid red 14 adsorption was found as 95.0%. In order to investigate the efficiency of adsorption, pseudo-first-order, pseudo-second-order and intra particle diffusion kinetic models were studied. It was observed that the pseudo-second-order kinetic model fits better than other kinetic models with good correlation coefficient. Equilibrium data were fitted to the Langmuir model. Adsorption equilibrium reach within 40 min of contact time and adsorption capacity was found to be 50.0 mg g-1. The results showed this nanocomposite can be used for the efficient removal of acid red 14 from different wastewater sample

A simple and rapid method for extraction and preconcentration of crystal violet in ???????? samples was developed by using dispersive liquid-liquid microextraction based on solidification of floating organic drop[1]. The mixture of extraction solvent and disperser solvent is rapidly injected into the sample solution. After injection, centrifugation is applied as in conventional DLLME to cause flotation of the organic phase at the top of the aqueous phase; this is then followed by cooling in a beaker containing ice in order to solidification of the floated organic droplet[2] and the amount of analyte is maesured with spectrophotometer. some parameters such as disperser solvent, extraction solvent,salt effect and extraction time were optimized.
 

In this study, the adsorption of Fuchsin and Malachite green (MG) dyes by molecular imprinted polymer (MIP) as adsorbent was reported. In the first research, a highly selective sample cleanup procedure combining molecular imprinting technique (MIT) and solid-phase extraction (SPE) was developed for the simultaneous determination of MG and Fuchsin in seawater and seafood samples by spectrophotometry. The effects of different parameters such as pH, volume of eluent, amount of sorbent on the extraction of both dyes were investigated and optimum conditions were established. Linear calibration curves were obtained in the range of 5-600 ng mL-1 for MG and 5-240 ng mL-1 for Fuchsin under optimum conditions. Limit of detection was 0.63 and 0.24 ng mL-1 for MG and Fuchsin, respectively. The relative standard deviation for ten replicate measurements of 200 ng mL-1 of MG and Fuchsin was 2.35 and 1.96 %, respectively. The method was applied to the simultaneous determination of the dyes in seawater and seafood samples.
In the second research, an easy removal method for Fuchsin from water samples by molecular imprinted polymer as an adsorbent has been reported. Response surface methodology (RSM) was employed to investigate the effect of different operating parameters on the uptake of Fuchsin using MIP. Optimized values of adsorbent dosage and pH for Fuchsin adsorption were found as 0.1 g, and 5, respectively. In order to investigate the mechanism of Fuchsin removal, various adsorption isotherms such as Langmuir, Freundlich and Temkin were studied. The experimental data revealed that the Freundlich isotherm gave a more satisfactory fit for Fuchsin removal. The Maximum adsorption capacity of adsorbent was 15.60 mg g-1. The adsorption process was rapid and obeyed pseudo-second order kinetic. The results showed this MIP can be used for the efficient removal of Fuchsin in seawater and seafood samples.
 

 The adsorption experiments were carried out in batch mode to optimize various parameters such as, contact time, pH, initial dye concentration, and temperature that influence the adsorption. The points of zero charge (PZC) for the nanocomposite was obtained and was about 6. The maximum adsorption capacity of adsorbent at pH=5 and contact time of 60 minutes was 113.64 mg.g−1. Langmuir, Freundlich, temkin and Dubinin-Radushkevich adsorption isotherm models were applied to describe isotherm constants. Equilibrium data agreed well with the Freundlich isotherm model. Thermodynamic studies revealed that the nature of sorption is spontaneous and endothermic. The obtained results showed that the this nanocomposite adsorbent can be reused for least 5 adsorption–elution cycles without any decreased in the efficiency. The removal efficiency of this nanocomposite toward sunset yellow dye is above 95% in distilled water, tap water, river water, wastewater and also food samples .

In the first research, SBA-15 mesoporous silica was synthesized under a moderately condition by sol gel methode. The adsorption experiments were carried out in batch mode to optimize various parameters such as, contact time, pH, initial dye concentration, and temperature that influence the adsorption. The points of zero charge (PZC) for the nanocomposite was obtained and was about 7. The maximum adsorption capacity of adsorbent at pH=5 and contact time of 40 minutes was 114.94 mg.g−1. Langmuir, Freundlich and temkin adsorption isotherm models were applied to describe isotherm constants. Equilibrium data agreed well with the Langmuir isotherm model and equilibrium data were better fitted by pseudo-second order kinetic model. The removal efficiency of this nanocomposite toward sunset yellow dye is above 95% in distilled water, tap water, river water and also food samples .

In the second research, an easy removal method for Tartrazine from water samples by a NanoTiO2/chitosan as an adsorbent has been reported. Response surface methodology (RSM) was employed to investigate the effect of different operating parameters on the uptake of tartrazine using chitosan/titana nanocomposite. Optimized values of adsorbent nanoparticle dosage and pH for tartrazine adsorption were found as 0.07 g , and 3, respectively. In order to investigate the mechanism of tartrazine removal, various adsorption isotherms such as Langmuir, Freundlich, Temkin were studied. The experimental data revealed that the Langmuir isotherm gave a more satisfactory fit for tartrazine removal. The maximum adsorption capacity of adsorbent was 61.35 mgg−1. The adsorption process was rapid and obeyed pseudo-second-order kinetics. The results showed this nanocomposite can be used for the efficient removal of tartrazine from distilled water, tap water, river water and also food samples

 Abstract:
In this study two methods were used for removal of Amaranth and Acid Red14 from wastewater samples. In the first research, the ability ofmultiwalled carbon nanotubesmodified byMnO_2 (NMnO2/MWCNTs) as an efficient adsorbent for the removal of Amaranth from water samples was investigated. In order to obtain maximum removal efficiencies, the effects of pH, kind of buffer, volume of buffer, amount of adsorbent, electrolyte, Standing time, volume of solution and interfering ions were investigated. Maximum removal efficiencies were obtained in acidic media.In order to obtain maximum capacity of the adsorbent, Langmuir adsorption isotherm in the optimum condition was plotted and it was illustrated that adsorbent capacity is 500 mg g-1. The experimental kinetic and equilibrium data were better fitted by pseudo-second order kinetic model and Freundlich isotherm. This method was successfully used for removal of Amaranth from spiked water samples such as tap water samples and food Samples.
In the second research, an easy removal method for Acid Red14 from wastewater samples by CuO modified by Tetra Buttyl Ammonium Bromide(TBAB) as an adsorbent is reported. The effects of various parameters on removal method such as pH, kind of buffer, volume of buffer, Standing time, amount of TBAB, amount of adsorbent, electrolyte, volume of solution, temperature and interfering ions were investigated. Maximum removal efficiencies were obtained at pH 4 and room temperature. The experimental kinetic and equilibrium data were better fitted by pseudo-second order kinetic model and Langmuir isotherm and maximum capacity of adsorbent is 42.55 mg g-1. This method was successfully used for removal of Acid Red14 from spiked water samples such as tap water samples and red powder used in textile industry.